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It takes 2 people to do this. One person has to carry a spotlight and showcase the flower. The other person aims the camera right on the flower. Use regular daytime settings, close-up macro and full flash. Turn the flashlight off and take the photo.
In order to get the rose in the background to show up...just set up the front flower, then have your assistant keep the flashlight on the background flower while you snap the shot.
Pretty cool photography!